Figure 6

A common molecular network for NBL aggressiveness, derived by meta-analysis, combining gene expression NBL databases from two different studies.

(a) Two microarray datasets were analysed: Cologne 2-Color (251 samples) and Essen Affymetrix (76 samples). In the network, the NME1 gene is the most connected. Nodes represent genes; node size represents the degree of the node (i.e., the number of connections it has). Nodes are connected with lines, which represent interactions between genes. Line thickness represents the strength of interactions, as measured by mutual information. Statistical confidence of the interactions is represented by the opacity of the color of the lines (strong gray, most reliable interactions; light gray, least reliable interactions). (b) Proteins extracted from tumor tissues and SH-SY5Y cells were loaded on acrylamide gels to determine the protein expression levels of TRIM22 and PTPRA. β-Actin was used as the loading control. Action view of gene networks of genes that are highly correlated with Trim22 (c, green) and PTPRA (d, green). Modes of action are shown in different colors. Genes highlighted in yellow were further investigated. (e) Western blotting showing CPP effects on the Akt pathway in SH-SY5Y cells. β-Actin was used as the loading control. (f) Western blotting for activated β-Catenin, c-Myc, P-Ikbα and Ikbα protein levels in SH-SY5Y cells after CPP overexpression. β-Actin was used as the loading control. (g) Following Ad-CPP infections in SH-SY5Y cells, protein levels of EGFR, Paxillin, Fak, Fak (Y397), Grb2 and Src (Y527) were analyzed by Western blotting. β-Actin was used as the loading control.