Table 1 Growth of strains. (a) Growth responses of liquid cultures after 16 hours in minimal glucose medium supplemented with phenylalanine, tyrosine, tryptophan, shikimate and pyridoxine. (b) Growth responses of liquid cultures in mineral glucose medium after 16 hours for the G1858 strain and 5 days for the other strains
From: A Metabolic Prototype for Eliminating Tryptophan From The Genetic Code
Growth | ||
|---|---|---|
Strain genotype | Mineral glucose medium containing Phe, Tyr, Trp, shikimate, pyridoxine (a) | Mineral glucose medium (b) |
G1851 ΔtktA ΔtktB ΔmutT | + | - |
G1858 ΔtktA ΔtktB ΔmutT p::tktY wt | + | + |
G1855 ΔtktA ΔtktB ΔmutT p::tktY His481Trp | + | - |
G2052 ΔtktA ΔtktB ΔmutT p::tktY His30Trp | + | - |
G1857 ΔtktA ΔtktB ΔmutT p::tktY His481Trp hisT:CCA | + | + |
G2140 ΔtktA ΔtktB ΔmutT p::tktY His30Trp hisT:CCA | + | + |
