Table 3 Kinetic parameters for wild-type and mutant transketolase
From: A Metabolic Prototype for Eliminating Tryptophan From The Genetic Code
Apparent Km (μM) | |||
---|---|---|---|
Yeast Transketolase | Xylulose 5P | Ribose 5P | kcat (s−1) |
Wild type | 127 ± 21 | 173 ± 23 | 24.60 ± 1.09 |
H481G mutant | 692 ± 380 | 37 ± 4 | 1.58 ± 0.18 |