Table 3 Kinetic parameters for wild-type and mutant transketolase

From: A Metabolic Prototype for Eliminating Tryptophan From The Genetic Code

 

Apparent Km (μM)

 

Yeast Transketolase

Xylulose 5P

Ribose 5P

kcat (s−1)

Wild type

127 ± 21

173 ± 23

24.60 ± 1.09

H481G mutant

692 ± 380

37 ± 4

1.58 ± 0.18