Table 1 List of primers developed and used in this study
From: Insect symbiont facilitates vector acquisition, retention and transmission of plant virus
Gene | Primer set | Expected size (bp) | Primer sequence (5′to 3′) | Reference |
|---|---|---|---|---|
Portiera 16S rDNA | Port-F | 229 | TAGTCCACGCTGTAAACG | |
| Â | Port-R | Â | AGGCACCCTTCCATCT | Â |
Hamiltonella 16S rDNA | Ham-F | 243 | GCATCGAGTGAGCACAGTT | |
| Â | Ham-R | Â | TATCCTCTCAGACCCGCTAA | Â |
TYLCV | C473 | 410 | AGTCACGGGCCCTTACA | |
| Â | V61 | Â | ATACTTGGACACCTAATGGC | Â |
TYLCV (q-PCR) | TY-F | 144 | GTCTACACGCTTACGCC | |
| Â | TY-R | Â | GCAATCTTCGTCACCC | Â |
β-actin | β-actin F | 130 | TCTTCCAGCCATCCTTCTTG | |
|  | β-actin R |  | CGGTGATTTCCTTCTGCATT |  |
25S Tomato | 25S rRNA F | 113 | ATAACCGCATCAGGTCTCCA | |
| Â | 25S rRNA R | Â | CCGAAGTTACGGATCCATTT | Â |
