Table 1 Cell physical parameters, methods and measurements
From: A physical sciences network characterization of non-tumorigenic and metastatic cells
Cell physical parameter | Technology name/ Physical method | Measurement |
|---|---|---|
Morphology | ||
Cell and nuclear shape, volume | 2D: Differential interference contrast microscopy | 2D: Shape; length |
| Â | 3D: Optical computed tomography | 3D: Volume; characteristic nuclear shape (nuclear sphericity parameter) |
Nuclear architecture | Partial wave spectroscopy | Nuclear disorder strength (Ld) |
Cell growth as a function of matrix stiffness | Immunofluorescence; confocal microscopy | Cell proliferation; cell morphology |
Cell surface | Total internal reflection fluorescence; epifluorescence microscopy | CD44 expression patterns; lipid raft distribution |
Motility and Mechanics | ||
Cell motility | 1D, 2D and 3D motility assays | Speed; radial displacement |
Cell adhesion and rolling | HA Micropatterns; flow chamber adhesion assay | Cell binding to HA pattern; rolling velocity |
Mechanical flexibility | Atomic force microscope-based nano-indentation | Elastic modulus |
Internal fluidity | Ballistic injection nanorheology | Mean square displacement |
Endogenous force generation | Traction force microscopy | Tension maps, force magnitudes |
Stress Response and Survival | ||
Hypoxic conditions | Microscopic imaging of 3D cultures (intracellular Ca2+ and DNA) | 2D Cell viability; 3D O2 consumption; CEA expression |
Chemical stress | As above | Cell viability |
