Figure 3

EGFP⁺ γ-pcdh deficient cells in the SVZ.

(A–C) Morphological comparison of EGFP⁺ and Tom⁺ cells in the SVZ of γ-pcdh^lox/lox injected mice at 4, 6 and 10 dpi. (D) Quantitative analysis of EGFP⁺, Tom⁺ and EGFP⁺/Tom⁺ double-positive cells in the SVZ at 4, 6 and 10 dpi. (E–G) Immunolabeling for EGFP and γ-pcdh expression in γ-pcdh^lox/lox injected mice at 6, 10 and 14 dpi, showing of EGFP⁺/γ-pcdh⁻ (green, white arrows), γ-pcdh⁺ (red, white arrows) and EGFP⁺/γ-pcdh⁺ (yellow, grey arrow) expression in single and merged layers. (H) Quantitative analysis of the percentage of EGFP⁺ cells in the SVZ at 4, 6, 10 and 14 dpi. (I–L) Immunolabeling for neural stem cell markers on parasagittal sections at 14 dpi showing co-expression of EGFP with GFAP (I, white arrows, inset SVZ), Sox2 (K, white arrows, inset SVZ) and Dcx (L, white arrows, inset SVZ). Scale bars in A–C, 50 μm, in E–G, 20 μm, in I–L 50 μm, in insets 100 μm.