Figure 7

Morphology of LV-infected EGFP⁺ and Tom⁺ olfactory GCs.

(A–F) Morphology of olfactory EGFP⁺ and Tom⁺ GCs analyzed at 14, 21 and 42 dpi. (A, D) A parasagittal section from γ-pcdh^lox/lox injected mice showing a reduction in dendritic arborization of EGFP⁺ cells at 14 dpi. Insets showing magnification with spines on Tom⁺ (A) and on EGFP⁺ (D) dendrites at 14 dpi. (B, E) Parasagittal section at 21 dpi showing a reduction of dendritic arborization in EGFP⁺ cells (E). Insets showing magnification of dendrites on Tom⁺ (with spines B) and of EGFP⁺ (E) dendrites at 21 dpi. (C, F) Parasagittal section at 42 dpi showing prominent dendrites and spines only in Tom⁺ GCs (C). Note that EGFP⁺ GCs (F) have shorter dendrites and lack spines. (G–I) Sholl analysis for dendrite morphology of EGFP⁺ and Tom⁺ olfactory GCs at 14, 21 and 42 dpi (wt in black, γ-pcdh ko cells in grey). (K–L) Bar-graphs showing quantitative analysis for length of apical dendrites (K), dendritic branching (L) and spine numbers (M). Scale bars in A–F 100 μm, in insets 10 μm.