Figure 2
From: Controlling Circadian Rhythms by Dark-Pulse Perturbations in Arabidopsis thaliana
Circadian response of Arabidopsis thaliana CCA1::LUC to application of a 2-h dark pulse under constant light.
(a) Bioluminescence signals were averaged over plants with (closed circle; n = 6) and without pulse perturbation (open circle; n = 40). The gray rectangle indicates the 2-h dark pulse. (b) Phase response curve for the dark pulse, indicating the phase shift of the plant circadian rhythm (n = 134) against phase Φ, at which the dark pulse was applied24. The solid line G(Φ) = −0.186 + 0.654 sin(Φ− 1.38) + 0.158 sin(2Φ − 1.669) shows a curve fitting using the first and second harmonics. (c) Amplitude response curve for the dark pulse, indicating the amplitude change of the plants (n = 169) against phase Φ of the pulse perturbation. The solid line represents −dG(Φ)/dΦ.
