Figure 1

12/15-LOX overexpression in naïve mouse leads to asthma features.

(A) Healthy Balb/c mice were administered either 12/15-LOX plasmid (12/15-LOX OE) or control plasmid (Control). (B–D) Western blot and ELISA to determine 12/15-LOX expression, levels of 13-S-HODE and 12-S-HETE in lung cytosol. (E) Airway resistance in response to increasing concentrations of methacholine as the percent baseline airway resistance assuming saline aerosol-derived values as baseline (n = 5–6 each group) was determined. Photomicrographs of bronchovascular regions stained with hematoxylin and eosin (F), periodic acid-Schiff (G) and Masson trichrome (H) stains of lung sections. I) Inflammation score was determined in lung sections. Data are representative of two independent experiments. Results are shown as quantile box plots for (13-S-HODE and 12-S-HETE) or as mean ± s.e.m (airway resistance and inflammation score). The line across the middle of the quantile box is the median; the ends of the box are 25th and 75th quantiles and the whiskers extending from either end of the box are the 10% and 90% quantiles. Significance is determined with unpaired Student t test or Mann-Whitney test (***P < 0.05).