Figure 2

12/15-LOX overexpression in naïve mouse leads to bronchial epithelial injury.

Mitochondrial complex IV activity (A), mitochondrial membrane potential (B), cytosolic cytochrome C levels (C) and cytosolic caspase 3 activity in lung cytosol (D). MITO, mitochondria. Photomicrographs of Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained lung sections (E) and the percentage of TUNEL-positive bronchial epithelium (F). (G) TEM images of lung sections. Red arrows indicate the normal mitochondria with well developed cristae and Red arrowheads indicate the abnormal mitochondria with loss of cristae and swelling. Results are shown as quantile box plots. The line across the middle of the quantile box is the median; the ends of the box are 25th and 75th quantiles and the whiskers extending from either end of the box are the 10% and 90% quantiles.