Figure 7
From: 12/15-lipoxygenase expressed in non-epithelial cells causes airway epithelial injury in asthma
IL-13-mediated epithelial injury is reduced by 12/15-LOX deficiency.
Murine rIL-13 was administered as shown in Materials and Methods. Lung mitochondria and cytosols were examined for activities of complex I & IV, mitochondrial membrane potential and cytochrome C (A–D). TUNEL apoptotic assay (E) and the percentage of TUNEL-positive bronchial epithelium (F). Arrows indicated the apoptotic bronchial epithelia. (G) Ultrastructural changes of bronchial epithelial mitochondria were analyzed by TEM in Balb/c mice. Lung mitochondria and cytosols were examined for activities of complex I and cytochrome C (H–I) in 12/15-LOX Knockout mice. Results are shown as quantile box plots. The line across the middle of the quantile box is the median; the ends of the box are 25th and 75th quantiles and the whiskers extending from either end of the box are the 10% and 90% quantiles.
