Figure 1
From: TAK1 regulates autophagic cell death by suppressing the phosphorylation of p70 S6 kinase 1
dTAK1 overexpression induces autophagy in Drosophila eye discs.
(A) Third-instar larval eye discs were stained with 1 μM LysoTracker Red. Hoechst staining of nuclei is shown in blue. Scale bars, 100 μm. (B) Quantification of the number of LysoTracker Red-positive punctate structures. The values represent the means ± s.d. of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001. (C) The transcript levels of dTAK1 in the third-instar larvae were analyzed by RT-PCR. Rp49 was used as an internal control. (D) The punctate structures containing GFP-Atg8a were used as markers of autophagosome formation in the eye discs of third-instar larvae. (E) Quantitative analysis of GFP-Atg8a puncta. The values represent means ± s.d. of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001.
