Figure 3
From: TAK1 regulates autophagic cell death by suppressing the phosphorylation of p70 S6 kinase 1
Autophagy-related proteins are involved in TAK1-induced GFP-LC3-II conversion and cell death.
(A–D) HEK 293T cells were transfected with 100 nM of the indicated siRNAs for 16 h and transfected with TAK1 for another 24 h. The expression levels of TAK1, GFP-LC3-I, GFP-LC3-II, Atg5, Atg7, Atg12, beclin1 and β-actin were analyzed by immunoblotting. (E) Cell viability of HEK 293T cells transfected with indicating siRNAs and TAK1 was measured by trypan blue assay. The values represent the means ± s.d. of three independent experiments. * P < 0.05.
