Figure 4
From: TAK1 regulates autophagic cell death by suppressing the phosphorylation of p70 S6 kinase 1
S6K1 is involved in TAK1-induced autophagy in vivo and in vitro.
(A) Eye discs of flies of the indicated genotypes were stained with 1 μM LysoTracker Red to detect autolysosomes. Autolysosomes were visualized as LysoTracker Red-positive punctate structures. Scale bars, 100 μm. (B) The numbers of autolysosomes present in flies of each genotype were quantified. The values represent the means ± s.d. of three independent experiments. (C) Ectopic expression of the indicated genes in Drosophila eyes was induced using the eye-specific driver GMR-GAL4. The adult eye phenotypes of the flies of the indicated genotypes were photographed. Scale bars, 0.1 mm. (D) HEK 293T cells were transfected with TAK1-WT for 24 h and then transfected with the indicated siRNAs for 48 h. Cell lysates were then harvested and analyzed by immunoblotting using the indicated antibodies. A GFP antibody was used to measure the levels of LC3-II, which are correlated with the number of autophagosomes.
