Figure 4 | Scientific Reports

Figure 4

From: Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes

Figure 4

Transcription efficiencies of cel7A-1 promoters from strains 114-2 and JU-A10-T.

Strains were grown in 1% (w/v) glucose medium or 1% (w/v) cellulose medium for 48 h. Transcription efficiency was analyzed by real-time quantitative RT-PCR of the reporter eGFP gene. Transcription levels of eGFP gene were normalized to 100-fold of those of β-actin. Error bars represent standard deviations of triplicate measurements. FM, fluorescence microscopy; LM, light microscopy. White bar = 10 μm.

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