Figure 1 | Scientific Reports

Figure 1

From: Intra-membrane Signaling Between the Voltage-Gated Ca2+-Channel and Cysteine Residues of Syntaxin 1A Coordinates Synchronous Release

Figure 1

Expressing GFP, wt Sx1A and Sx1A mutant Sx1AC271V, Sx1AC272V and Sx1AC145A by SFV vectors in bovine chromaffin cells.

(a) Chromaffin cells were infected with SFV vectors of GFP, RFP-Sx1A wt, RFP-Sx1ACC/VV, RFP-Sx1AC271V, RFP-Sx1AC272V and RFP-Sx1AC145A. The cells were visualized by confocal microscopy (b) Western blot analysis of Sx1A expression was determined in cell lysates from chromaffin cells infected with SFV-GFP, RFP-wt Sx1A, RFP-Sx1ACC/VV (upper), RFP-Sx1AC271V or RFP-Sx1AC272V or RFP-Sx1AC145A (lower) (c) Quantification of the relative expression of RFP-wtSx1A and RFP-Sx1A mutants to endogenous Sx1A present in the cells.

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