Figure 3 | Scientific Reports

Figure 3

From: Intra-membrane Signaling Between the Voltage-Gated Ca2+-Channel and Cysteine Residues of Syntaxin 1A Coordinates Synchronous Release

Figure 3

Sx1A mutants Sx1AC271V and Sx1AC272V affect secretion rate in bovine chromaffin cells.

(a) Sample amperometry traces for GFP and RFP-Sx1AC271V, RFP-Sx1AC272V and RFP-Sx1AC145A mutants elicited by a puff of K60 (arrow) (b) Left, Cumulative spike counts were plotted versus time to illustrate the time course of fusion triggered by depolarization (starting at t = 10). Right, expanded view of the initial cumulative spike counts. The mean frequency of the initial rate was calculated as the maximum slope in plot b left during the first 20 s of K60 stimulation (Table II) (c) Left, average number of spikes per cell (Table II), Middle, the average area underneath the spikes represents the total mean charge (total CA secretion) and is presented as the percentage of average secretion per cell. Right, the mean frequency of the initial rate was calculated as the maximum slope in plot b right, during the first 30s of recording. **p < 0.05; ***p < 0.005.

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