Table 1 Spike number, initial rates and total CA release induced by a 10 sec puff of K60 in bovine chromaffin cells infected with GFP, Sx1A wt and Sx1ACC/VV

From: Intra-membrane Signaling Between the Voltage-Gated Ca2+-Channel and Cysteine Residues of Syntaxin 1A Coordinates Synchronous Release

 

Cell (n)

Spikes (n)

Initial rate (Spikes/sec)

Initial secretion (pC)

Spikes/cell (n)

Spikes/cell (Inhibition) a (%)

Control

21

581

0.73 ± 0.03

14.6 ± 2.1

27.7 ± 2.4

-

pSFV GFP

32

721

0.80 ± 0.04

11.7 ± 1.6

22.5 ± 3.1

-

pSFV Sx1A wt

23

524

0.71 ± 0.03

11.5 ± 1.1

22.8 ± 2.4

0

pSFV Sx1ACC/VV

32

350

0.40 ± 0.01

6.0 ± 0.8***

10.9 ± 0.8***

52

  1. ***p < 0.005
  2. aPercent inhibition compared to GFP-infected cells.
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