Figure 4
From: Oxymatrine Prevents NF-κB Nuclear Translocation And Ameliorates Acute Intestinal Inflammation
Oxymatrine inhibits p65 nuclear translocation.
(a) IEC-6 cells were treated with 5 μg/mL LPS ± 4 mg/mL OMT for 30 minutes. Cells were fixed, permeabilized and immunofluorescent staining performed for p65 (inner panels). Hoescht nuclear staining was also performed (outer panels). Representative of 3 independent experiments. (b) p65 cytosolic and nuclear quantifications were measured as p65 nuclear accumulation in 3 random fields of view per slide. Graph represents combined data from 3 independent experiments. (c) Chromatin immunoprecipitation (ChIP) analysis of p65 binding to the Cxcl2 promoter of IEC-18 cells stimulated with LPS ± OMT for 30 and 60 min. PCR amplification products were separated using agarose gel electrophoresis and gel scan results are presented in inverse color. Representative of 2 independent experiments. **P < 0.01 compared to LPS control.
