Figure 4

Excluded volume histograms for all three SAP97 PDZ2 domains (wild type (WT), V388A and L322A) each at four different applied voltages.

The natively folded state was defined as the full width half max of the most stable protein at the lowest applied voltage (200 mV). This criterion was then applied to all other domains at all voltages in order to classify proteins as folded or unfolded. Data was collected with pores 15 ± 2 nm in diameter for each domain: wild type SAP97 PDZ2 (n_200mV = 202, n_400mV = 1005, n_600mV = 1474, n_800mV = 1006), V388A (n_200mV = 1719, n_300mV = 703, n_600mV = 768, n_800mV = 1493), L322A (n_200mV = 233, n_400mV = 674, n_600mV = 607, n_800mV = 550).