Figure 3 | Scientific Reports

Figure 3

From: A multisite blinded study for the detection of BRAF mutations in formalin-fixed, paraffin-embedded malignant melanoma

Figure 3

Results for sample P33 showing cross reactivity between the V600K probe (orange line) and a V600E mutation (blue) in CAST-PCR (D).

In this case the dCt value for the V600K assay was lower than the threshold value of 9.96, however Sanger sequencing (A) clearly showed this to be a V600E mutation. The results for SSCA (B) and HRM (C) also suggest a single V600E mutation.

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