Figure 2

Fewer lymphocytes infiltrate the CNS in CD146^EC-KO mice.

(a) Flow cytometry analysis revealed a reduction in the number and percentage of infiltrated leukocytes (CD45^highCD11b⁻) in the CNS of CD146^EC-KO mice compared to WT mice with EAE (n = 5 mice/group). (b) Flow cytometry analysis of the proportion of CD4⁺ and CD8⁺ cells in CD45^highCD11b⁻ cells and T_H1 and T_H17 lymphocytes in CD4⁺ cells in the CD146^EC-KO and WT mice with EAE (n = 5 mice/group). (c and d) Flow cytometry analysis of the percentage of CD146⁺ cells in the CD4⁺ and CD8⁺ cells (c) and T_H1 and T_H17 (d) cells in the CNS of CD146^EC-KO and WT mice with EAE (n = 5 mice/group). The data represent three independent experiments. *, p < 0.05, **, p < 0.01, ***, p < 0.001, n.s., not significant.