Figure 5
Decreased mRNA expression of different transcription factors and cytokines involved in TH17 differentiation after immunotherapy (SIT) in T-bet(−/−) mice.
(a) Experimental design of the murine asthma model with immunotherapy.Mice received 100 μg OVA/Alum intraperitoneally (i.p.), 1000 μg OVA subcutaneous (s.c.) and 2 mg/ml OVA intranasally (i.n.). (b–j) mRNA expression of Il-6 (b), Rorγt (c), Batf (d), Irf4 (e), Il-21 (f), Il-21R (g), Il-17A (h), Il-10 (i) and Il-2 (j) in lung tissue of T-bet(−/−) mice before and after SIT (n = 3–6 mice per group). Results in this figure are expressed as mean ± s.e.m. Students t test was used to calculate statistical significances in this figure. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.
