Figure 2
From: S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis
NO inhibits the phosphorylation of ERK and promotes MCF-7 cells apoptosis.
(A) Cells were treated with 1 mM SNP and the phosphorylation of ERK was examined by immunoblots. (B) ERK phosphorylation was quantified in cells treated with SNP (0 to 2 mM). (C) Wild type and constitutively active mutant of ERK (TEY-EED) were used to investigate the inhibitory role of NO on ERK phosphorylation. (D) Apoptotic effects were examined using flow cytometry.
