Figure 3
From: S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis

NO induces S-nitrosylation of ERK.
(A) Extracts from 293T cells treated with Cys-NO in the presence or absence of ascorbate and the level of ERK-SNO was detected by S-nitrosylation (Immuno-precipitation) assay in vitro. (B) 293T cells were treated with SNP (0 to 2 mM) and the level of ERK-SNO was detected by S-Nitrosylation Assay (Biotin-Switch).