Figure 5
From: S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis
S-nitrosylation of ERK1 directly inhibits ERK phosphorylation and promotes apoptosis.
(A) MCF-7 cells were transfected with ERK1-HA or ERK1 mutant form (C183A), the phosphorylation of ERK was determined by immunoblots. (B) ERK kinase activity was detected using the ERK kinase assay kit. P = 0.0022 (C) PPARγ, a phosphorylation target of ERK was examined for phosphorylation status. (D) MCF-7 cells were transfected with either wild type or C183A mutant ERK and the process of procaspase-9 was quantified by immunoblots. (E) Apoptotic effects of SNP in MCF-7 cells transfected with either wild type or C183A mutant ERK were determined by flow cytometry.
