Figure 4 | Scientific Reports

Figure 4

From: Microtubule Alterations Occur Early in Experimental Parkinsonism and The Microtubule Stabilizer Epothilone D Is Neuroprotective

Figure 4

MPTP affects MT stability in dopaminergic terminals.

(a) Immunoblot of levels of tyrosinated tubulin (Tyr Tub), detyrosinated tubulin (deTyr Tub) and acetylated tubulin (Ac Tub) in lysates of striatum of mice treated as in Fig. 1. (b) Densitometric analyses of immunoblot reported in a (mean ± S.E.M., n = 4–6 mice per group). For the quantitation, values of each α-tubulin PTM were normalized on the level of α-tubulin (α Tub) of the relative sample. *P < 0.05; one-way ANOVA, Dunnett post hoc test versus saline-injected mice. (c) Confocal images of striatum of mice treated as in Fig. 1. Green represents TH staining and red signals the various tubulin PTMs. Scale bar = 50 μm. hpi = hours post last injection of MPTP.

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