Figure 5 | Scientific Reports

Figure 5

From: Microtubule Alterations Occur Early in Experimental Parkinsonism and The Microtubule Stabilizer Epothilone D Is Neuroprotective

Figure 5

MPTP affects MT stability in dopaminergic neurons.

(a) Immunoblot of levels of tyrosinated tubulin (Tyr Tub), detyrosinated tubulin (deTyr Tub) and acetylated tubulin (Ac Tub) in lysates of substantia nigra of mice treated as in Fig. 1. (b) Densitometric analyses of immunoblot reported in a (mean ± S.E.M., n = 4–6 mice per group). For the quantitation, values of each α-tubulin PTM were normalized on the level of α-tubulin (α Tub) of the relative sample. *P < 0.05; one-way ANOVA, Dunnett post hoc test versus saline-injected mice. (c) confocal images of substantia nigra of mice treated as in Fig. 1. Green represents TH staining and red signals the various tubulin PTMs. Scale bar = 50 μm. hpi = hours post last injection of MPTP.

Back to article page