Figure 6
Repeated systemic injections of EpoD attenuate MPTP toxicity in mice.
(a) Biochemical analyses of striatal dopamine, DOPAC and HVA levels in C57Bl mice injected with MPTP (30 mg/kg, i.p., single injection) alone or in combination with EpoD (1 or 3 mg/kg, i.p.) dissolved in DMSO and injected 30 min before MPTP. Mice were killed 7 days later (mean ± S.E.M., n = 8–10 mice per group). *P < 0.05; one-way ANOVA, Dunnett post hoc test versus saline-injected mice. (b) Biochemical analyses of striatal dopamine, DOPAC and HVA levels in C57Bl mice injected with MPTP (30 mg/kg, i.p., single injection) alone or in combination with EpoD (1 mg/kg, i.p.) dissolved in DMSO and injected 30 min before MPTP and then for the following 4 days once a day. Mice were killed 7 days later (mean ± S.E.M., n = 8–10 mice per group). *, # P < 0.05; one-way ANOVA, Dunnett post hoc test versus saline-injected mice (*) or versus mice injected with MPTP alone (#). (c) Immunohistochemical analysis of TH in the pars compacta of substantia nigra of mice injected with a single i.p. injection of 30 mg/kg of MPTP, alone or combined with EpoD (1 mg/kg, i.p., 30 min prior to MPTP and then for the following 4 days, once a day). Scale bar = 250 μm. (d) Stereological counts of TH-positive cell in the substantia nigra pars compacta (mean ± S.E.M., n = 5 mice per group). *, # P < 0.05; one-way ANOVA, Dunnett post hoc test versus saline-injected mice (*) or versus mice injected with MPTP alone (#).
