Figure 7
EpoD rescues MT system in MPTP-treated mice.
(a) Immunoblot of levels of tyrosinated tubulin (Tyr Tub), detyrosinated tubulin (deTyr Tub) and acetylated tubulin (Ac Tub) in lysates of striatum of mice treated as in Fig. 6. (b) Densitometric analyses of immunoblot reported in a (mean ± S.E.M., n = 5 mice per group). For the quantitation, values of each α-tubulin PTM were normalized on the level of α-tubulin (α Tub) of the relative sample. *, # P < 0.05; one-way ANOVA, Fischer LSD post hoc test versus saline-injected mice (*) or versus mice injected with MPTP alone (#). (c) Immunoblot of levels of tyrosinated tubulin (Tyr Tub), detyrosinated tubulin (deTyr Tub) and acetylated tubulin (Ac Tub) in lysates of substantia nigra of mice treated as in Fig. 6. (d) Densitometric analyses of immunoblot reported in c (mean ± S.E.M., n = 5 mice per group). For the quantitation, values of each α-tubulin PTM were normalized on the level of α-tubulin (α Tub) of the relative sample. *, # P < 0.05; one-way ANOVA, Fischer LSD post hoc test versus saline-injected mice (*) or versus mice injected with MPTP alone (#). Tubulin dimers (Dim) and MT polymers (MT) of corpus striatum and substantia nigra were analyzed by (e) immunoblot and (f) densitometric analyses and are shown as ratio (mean ± S.E.M., n = 3 mice per group). *, # P < 0.05; one-way ANOVA, Fischer LSD post hoc test versus saline-injected mice (*) or versus mice injected with MPTP alone (#).
