Figure 1

IRGM/IRGM1 is highly expressed by foam cells in the lesion of atherosclerosis.
(a) AS plaque of ApoE-/- mice was validated by oil-red staining. IRGM1 expression in aortic arch of the mice with or without western diet was detected by western blot (b, c) (p = 0.0003, n = 5) and located by immuno-fluorescence staining (IRGM1-red, CD68-green, DAPI-blue) (d). Human IRGM expression in autopsy AS lesion was detected by immunohistochemistry staining (e). Data are represented as mean ± SEM.