Figure 3
Loss of IRGM/IRGM1 decreases oxLDL uptake by macrophage.
Irgm1+/+ or Irgm1−/− mice were fed with western diet for 3 months. Aorta was isolated and stained with red oil. (a) The representative and the quantified data of atherosclerotic lesion (n = 6/group, p = 0.04). BMMΦ were isolated from either Irgm1+/+ or Irgm1−/− mice. Ox-LDL or Dil-oxLDL was added for 48 hrs after isolation or induction. (b) Biochemical lipid quantitive assay was used to measure total cellular lipid. Dil-oxLDL was detected by either (c) confocal microscope (n = 4/group, p = 0.0004) or (d) flow cytometry (n = 8/group, p = 0.001). Human monocyte derived macrophage (HMMΦ) was transfected with either control-siRNA or IRGM-siRNA. IRGM-siRNA was validated by real-time PCR (, n = 3, p = 0.02). Dil-oxLDL uptake was measured by flow cytometry (f, n = 3, p = 0.04). Data are represented as mean ± SEM.
