Figure 4
Loss of tubular β-catenin does not affect the size of renal myofibroblast population.
(a) Quantitative determination of α-SMA mRNA expression in the obstructed kidneys at 7 days after UUO by qRT-PCR (n = 5). (b, c) Western blot analyses demonstrate no significant difference in renal α-SMA protein expression in Ksp-β-cat-/- and control mice at 7 days after UUO. Representative Western blot (b) and quantitative data (c) are shown (n = 5). (d) Immunohistochemical staining demonstrate little difference in the α-SMA-positive myofibroblasts in Ksp-β-cat-/- and control mice. Representative micrographs are presented. Scale bar, 50 μm. (e, f) Western blot analyses demonstrate that there was no difference in α-SMA protein expression in the obstructed kidneys of Ksp-β-cat-/- and control mice at 3 days after UUO. Representative Western blot (e) and quantitative data (f) are shown. Numbers (1, 2, 3 and 4) denote each individual animal in a given group.
