Figure 3
From: Attachment-regulated signaling networks in the fibroblast-populated 3D collagen matrix
Effect of FBS or PFT-μ on matrix cell number in the attached vs. released FPCM.
Attached matrices were incubated with 5% FBS for 48–72 h. The medium then was changed (DMEM with indicated FBS concentration in panel A and 5% FBS/DMEM in panel B), PFT-μ was added at indicated concentration for panel B, half of the matrices were released and cell/matrix was determined 48 and 72 h later. Each bar represents the mean ± sd of 12 hemacytometer counts from a single matrix; *p < 0.05, ANOVA (comparing the underlying four bars).
