Figure 1
From: Intravital analysis of vascular permeability in mice using two-photon microscopy

Bloodstream visualization with fluorescent-dextran.
(A) FITC-dextran injection first delineated arterioles (arrows) and then capillaries and postcapillary venules (arrow heads). The merged image depicts vessels at 0 min (red) and 1 min (blue) after the injection, respectively. (B) The black-and-white images (left) were converted to rainbow color-scale images (right) according to fluorescent intensity. (C) The blood vessel area (left: red) and the interstitial space (right: cyan) were separately circumscribed to monitor the fluorescent intensity.