Figure 2
From: Intravital analysis of vascular permeability in mice using two-photon microscopy
Vascular permeability under basal conditions.
(A) Sequential images taken 0, 10 and 60 min after FITC-dextran injection. The molecular sizes of the injected dextran are shown in the bottom left of each image. (B–D) Kinetics of the MFI in the blood vessel area (red) and interstitial space area (blue) following injection of 20 kDa , 40 kDa and 70 kDa dextrans, respectively. All experiments were performed at least three times with similar findings. (E, F) Fold change of the MFI in the interstitial space at 10 min or 60 min after the injection of FITC-dextran (20–150 kDa) or albumin. MFI in the interstitial space at 1 min was used as basis. *P < 0.05. (G) Sequential images of FITC-albumin injection. Scale bar = 100 μm.
