Figure 3
From: Intravital analysis of vascular permeability in mice using two-photon microscopy
Vascular hyper-permeability induced by injection of histamine.
(A, B) Sequential images before (left) and 5 min after (right) histamine injection. Mice were pretreated with an antihistamine agent, bepotastine besilate or vehicle 1 hour before injection. (C) Kinetics of the MFI in the blood vessel area (red) and in the interstitial space (blue). The arrow denotes the timepoint of histamine injection. Mice were pretreated with bepotastine (closed circles) or vehicle (open circles). (D) Fold changes of the MFI in the interstitial space 5 min after the injection of vehicle or histamine with or without antihistamine pretreatment. MIF at 1 min before the injection was used as basis. *P < 0.05. (E) Sequential images before (left) and 5 min after (right) histamine injection. The molecular sizes of the injected dextran are shown in the bottom left of each image. His; histamine, Bepo; bepotastine besilate. Scale bar = 100 μm.
