Figure 4
From: Intravital analysis of vascular permeability in mice using two-photon microscopy
Vascular hyper-permeability in models of anaphylaxis and CHS.
(A) Passive anaphylaxis model. Anti-TNP IgE antibody was injected intraperitoneally into mice. The following day, vascular permeability was analyzed in the ear after an intravenous injection of OVA-TNP. (B) Sequential images 1 and 5 minutes after the injection of OVA and OVA-TNP with or without bepotastine in the passive anaphylaxis model. In the rightmost panel, OVA-TNP was injected into WBB6F1-kitW/Wv mice. (C) Fold change of the MFI in the interstitial area 5 min after the OVA or OVA-TNP injection. MIF at 1 min before the injection was used as basis. *P < 0.05. (D) Serial images of the bloodstream in CHS-induced inflamed skin. The arrow indicates transient hyper-permeability in a localized area. Elapsed time is shown in the bottom right of each image. (E) Kinetics of the MFI in the interstitial space in (D). The dotted line represents the predicted baseline in this area.
