Figure 2

Immunohistochemical expression patterns of hEMSCPCs over 30 passages.

(A): Some cells at P2 showed nestin positive immunoreactivity (brown color). (B): Almost all cells at P2 were vimentin positive (brown color). (C): Almost all cells at P10, P20 and P30 showed nestin immunoreactivity (brown color). (D): Almost all cells at P10, P20 and P30 were vimentin immunoreactive (brown color). (E): At P2, P10, P20 and P30, a small number of cells were CK19 immunoreactive (brown color). (F): At P2, P10, P20 and P30, a limited number of CD10 immunoreactive cells were observed (brown color). (G): At P2, P10, P20 and P30, a few individual cells were CD3 immunoreactive (brown color). (H): At P2, no CD31-positive or VEGF R2-positive cells were seen. (I): At P10, P20 and P30, almost all cells expressed CD90 (green fluorescence). (J): At P2, P10, P20 and P30, most cells expressed CD73 (green fluorescence). (K): At P2, P10, P20 and P30, a fraction of cells expressed CD105 (green fluorescence). (L): At P10, P20 and P30, some specimens had no HLA-DR-positive cells. (M): At P10, P20 and P30, a small number of HLA-I-positive cells were observed in some specimens (green fluorescence).