Figure 3

Flow cytometry analysis of cell marker expression over time in culture.

Flow cytometry plots for various markers at P2, P10, P20 and P30. (A): CD73 was always highly expressed at P2, P10, P20 and P30; CD90 was moderately expressed at P2 and highly expressed at P10, P20 and P30; CD105 was moderately expressed at P2, P10, P20 and P30. (B): Substantial vimentin expression was observed at P2, P10, P20 and P30. Nestin expression mirrored CD90 expression. Nestin expression was moderate at P2 but high at P10, P20 and P30, whereas GFAP and β-III tubulin showed minimal expression throughout the culture period. (C): Human EMSCPCs expressed very low levels of CD34, CD45, CD3, CD19 and CD16. (D): CD10 expression was always low, while CK19, CD31 and VEGF R2 were barely detectable. (E): The tissue compatibility antigens HLA-DR and HLA-I were moderately or minimally expressed at P2; with increasing number of passages, fewer and fewer cells expressed these markers. No HLA-DR-positive cells were detected in some specimens at P10, P20 and P30.