Figure 3

DosD regulates the transcription and secretion of bpfA.
(A) The transcription levels of bpfA and aggA in ΔdosD relative to WT. The quantity of cDNA was normalized to the abundance of 16S cDNA in the qRT-PCR analysis. (B) The level of BpfA in ΔdosD bpfA+::gfp and bpfA+::gfp. WT was set as a control. (C) Localization and abundance of BpfA inΔaggA bpfA+::gfp and bpfA+::gfp. M indicates the sample of membrane fractions and C indicates the sample of cytoplasm fraction.