Table 1 Efficiency of CMAH targeting in fibroblast cells using ZFN and donor DNA. Female cells showed decrease efficiency of the targeting event
From: Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs
Source of cells | Source of ZFN | No. of cells transfected | No. of neo-positive colonies analyzed by PCR | No. of positive colonies (%) |
|---|---|---|---|---|
Male | DNA | 5 × 106 | 64 | 19 (29.7) |
mRNA | 5 × 106 | 48 | 15 (31.3) | |
Sub-total | 112 | 34 (30.4) | ||
Female | DNA | 5 × 106 | 39 | 3 (7.7) |
mRNA | 5 × 106 | 62 | 2 (3.2) | |
Sub-total | 101 | 5 (5.0) | ||
Total | 213 | 39 (18.3) |
