Table 2 Efficiency of CMAH targeting using fibroblast cells with isogenic CMAH profile as the reference sequence. No difference was found in the efficiency of targeting in male and female cells
From: Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs
Source of cells | No. of cells transfected | No. of neo-positive colonies | No. of positive colonies (%) |
|---|---|---|---|
Male | 2 × 106 | 13 | 5 (38.4) |
Female | 2 × 106 | 23 | 10 (43.5) |
Total | 36 | 15 (41.7) |
