Figure 2

Upper: FRET-melting curves^a for experiments carried out with hTel, c-myc, bcl2 and duplex DNA separately with the three compounds (dptmp, 1 and 2). Lower: dose-dependent inhibition of PCR amplification by the different concentrations of the compounds (dptmp, 1 and 2) in the PCR-stop assays^b.

^a All experiments were conducted at a DNA concentration of 400 nM and with a 0.5 μM concentration of the compounds, in Tris-HCl buffer (10 mM, pH 7.4) containing 60 mM potassium cacodylate buffer (DNA sequence: black; red: dptmp; blue: 1, green: 2). ^b The amplified PCR products were then analyzed on 15% non-denaturing polyacrylamide gels (100 V, 30 min) in 1× TBE buffer, followed by silver staining.