Figure 4
TRAP assaya results for the three compounds (dptmp, 1 and 2), showing telomeric ladders produced by PCR amplification of the oligonucleotides generated by the action of telomerase on a TS primer. The lower band is an internal control primer (ITAS).
a Each single assay was dependent on 1.0 μL of telomerase extract (200 cells), prepared from HeLa cells with NP-40 lysis buffer. Negative controls 1 and 2, which accompanied every assay, involved either incubating approximately 1.0 μL of cell lysate at 85°C for 10 min before primer extension or incubating approximately 1.0 μL of NP-40 lysis buffer, respectively. The control (0 nM) assay was performed without adding any compound.
