Figure 3
From: Zebrafish crypt neurons project to a single, identified mediodorsal glomerulus
TrkA antibody labels a single glomerulus in the olfactory bulb.
(a), (d), (f) Whole mount of adult zebrafish olfactory bulb labeled with (a) TrkA antibody (green); (d) synaptic vesicle protein 2 (SV2, red); and (f) overlay of both labels. SV2 is a general synaptic marker, which visualizes all glomeruli. The olfactory nerves were cut at the entrance to the olfactory bulb (asterisks) before staining. Dorsal view, for orientation compare the schematic drawing (g). A single labeled glomerulus (arrowhead), very far posterior and ‘not-quite-medial’, is seen in each olfactory bulb, entered by two nerve fascicles (arrows). Yellow color in overlay (f) confirms the TrkA-labeled structure as glomerulus. (b), (c), higher magnification of two other TrkA-labeled glomeruli, each with two incoming nerve fascicles. (e) posterior vibratome cross section of olfactory bulbs shows the extremely dorsal position of the TrkA-labeled glomerulus. No other glomeruli are labeled by TrkA antibody in this or more anterior bulbar sections (Suppl. Fig. 1c). Scale bar 200 μm for (a,d,f) and 20 μm for (c,e).
