Figure 5
From: Zebrafish crypt neurons project to a single, identified mediodorsal glomerulus
Quantitative analysis of tracing results shows specific crypt neuron labeling for mdg2 injection sites.
(a) Scatter plot of vertical vs. horizontal diameter for injections into mdg2 (left panel) and dlg1 (right panel), each cross represents a single cell, data are from the same injections depicted in Fig. 4. Cells are considered to have crypt-like morphology, if the ratio vertical to horizontal diameter is equal or less than 1.5 (cutoff visualized by the magenta line). Note the dense cluster of crypt neurons in the mdg2 injection and the absence of crypt neurons for injections into the dlg position. (b) histogram and (c) CDF of the diameter ratios for mdg2 (red) and dlg1 (blue) injections and trkA-labeled cells (green, same values as shown in Fig. 1f). Note that distributions for mgd2 injections and TrkA, the crypt neuron marker, are indistinguishable, whereas the distribution for dlg1 sharply diverges. (d) histogram and (e) CDF of the basal-to-apical position for mdg2 (red) and dlg1 (blue) injections and trkA-labeled cells (green), same conclusion as in (b–c). (f) Percentage of cells with crypt-like diameter ratios in TrkA-labeled cells, cells backtraced from the mdg2 injection and the dlg1 injection. (g) schematic representation of the backtracing results.
