Figure 6
Microtubule structure in the HeLa cell stained by a fluorescent dye (Alexa 488): (a) diffraction-limited image; reconstruction images using (b) the Lucy-Richardson deconvolution method and (c) the proposed method from 500 speckle illuminations; and (d) line profiles of the dotted areas in (a–c).
The light-blue line is from a diffraction-limited image; the dark blue line is from the Lucy-Richardson deconvolution; the red line is from the proposed method. Note that the proposed image can resolve a distance of 84 nm, which corresponds to threefold improvement in the resolution over the diffraction limit. The scale-bars are 500 nm in (a,b) and 250 nm in (c,d).
