Figure 7
Experimental image system constructed through modifications of the epi-fluorescence microscopy.
The excitation laser (532 nm or 488 nm) is combined by the dichroic mirror (DM1) and scattered by the diffuser (D1) in front of the illumination optics (LI, L1 and L2); then, the scattered light is focused on the sample plane by the objective lens (O). The emission fluorescence signal returns through the dichroic mirror (DM2) and the emission filter (F); it is also magnified by the tube lens (L3) in front of an EMCCD camera (D2).
