Figure 3
From: p38γ overexpression in gliomas and its role in proliferation and apoptosis
Reduction of p38γ expression in U251 cells by siRNA.
(a) RNAi leads to the down-regulation of p38γ mRNA level. ‘p38γ/siRNA’ refers to p38γ silencing group; ‘BC’ refers to blank control group; ‘NC’ refers to negative control siRNA-treated group. (b & c) RNAi leads to down-regulation of p38γ and hTERT protein expression in three groups. The band intensity ratio of p38γ/GAPDH in p38γ/siRNA-treated cells (0.20 ± 0.015) was significantly down-regulated compared with that in NC cells (0.59 ± 0.004) and BC U251 cells (0.58 ± 0.011). As to hTERT/GAPDH, the dates were 0.20 ± 0.020 (p38γ silencing group), 0.59 ± 0.013 (BC) and 0.57 ± 0.012 (NC). Full-length blots are presented in Supplementary Figure S2.
